Part:BBa_K1684001
amilGFPK12
Yellow chromoprotein optimized for E.coli k12
Usage and Biology
Chromoproteins are a collection of proteins that express pigments of strong colors. The AmilGFP derivate from Acropora millepora, present a yellow pigment when it’s expressed. Also, the protein has a maximum absorption of 512 nm.
This version is codon optimized for the expression in E.coli K12.
Results
The Figure 1 presents a 1% agarose gel which contains two samples, by triplicate, next to the ladder. In line 4,5 and 6 are repeated samples of the piece amilGFP optimized for E. coli K12 (BBa_K1684001). For both BioBricks, the electrophoresis results correspond to the theoretical size, greater than 2500 bp and less than 3000 bp; also it can be seen that there is no variation between samples, replicas, indicating the reliability of E. coli K12 transformations.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 612
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Alieva NO, Konzen KA, Field SF, Meleshkevitch EA, Hunt ME, et al. (2008) Diversity and Evolution of Coral Fluorescent Proteins. PLoS ONE 3(7): e2680. doi:10.1371/journal.pone.0002680
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